MECHANISMS OF PARASYMPATHETIC INFLUENCES ON THE HEART IN THE DEVELOPMENT OF OF THE EFFECT ANTIISCHEMIC DISTANT CONDITIONING MYOCARDIUM

The aim of the study: to investigate the involvement of distinct parasympathetic and sympathetic beta-adrenergic mechanisms in infarct-limiting effect of remote ischemic conditioning. Materials and methods. Experiments have been conducted on white male rats. Remote ischemic conditioning was performed as 15-min bilateral femoral arteries occlusion before the onset of myocardial ischemia (RIPC, n=8), on the 10-th minute of ischemia (RIPerC, n=8) or on the 10-th minute of reperfusion (RIPostC10', n=8). To study the role of vagal nerves in the development of remote ischemic conditioning femoral arteries occlusion was performed in conditions of bilateral vagotomy (Vagotomy+RIPC, n=8; Vagotomy+RIPerC, n=7; Vagotomy+RIPostC10', n=8). M-cholinoreceptors were blocked by atropine (groups Atropine+RIPC, n=10; and Atropine+RIPostC10', n=6). Beta-adrenoreceptors blockade was induced by injection of metoprolol or atenolol on the 1-st minute of reperfusion to animals with intact vagal nerves (groups MetR+RIPostC10', n=10; and AtenR+RIPostC10', n=10) or after bilateral vagotomy (groups Vagotomy+MetR+RIPostC10', n=10; Vagotomy+AtenR+RIPostC10', n=10). Results. RIPC, RIPerC и RIPostC10' limited infarct size by 56%, 58% and 49% respectively (all p<0,001 vs Control). Bilateral vagotomy, performed before the onset of myocardial ischaemia, abolished infarct-limiting effect of RIPC and RIPerC, but had no effect on cardioprotection induced by RIPostC10'. Atropine administration abolished cardioprotective effect of both RIPC and RIPostC10'. I.v. infusion of metoprolol or atenolol to the animals with intact vagal nerves had no effect on cardioprotection induced by RIPostC10', but administration of these agents in combination with vagotomy abolished this phenomenon (p>0,05 vs Control, p<0,001% vs RIPostC10'). Conclusions. Cardioprotective effect of RIPC and RIPerC is mediated mainly by vagal control of heart, whereas in RIPostC10' beta-adrenoreceptors are also important. Meanwhile, М-cholynoreceptors stimulation is a key point in the development of both RIPC, as well as RIPostC10'.

дистантное ишемическое кондиционирование миокарда, парасимпатическая иннервация сердца, ишемия и реперфузия миокарда, remote ischaemic heart conditioning, parasympathetic heart innervation, myocardial ischaemia and reperfusion

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